Preliminary
Data Regarding Polyphenols, Carotenoids and Flavonoids Content Correlated with
Antioxidant Activity of Some Taraxacum
Sp. Fluid
Extracts
Maria-Virginia TĂNASĂ (ACREȚEI)1*, Ticuța NEGREANU-P RJOL2*,
Carmen CHIFIRIUC3 , Dan
Răzvan POPOVICIU4 ,
Larisa ANGHEL (CIREAȘĂ)5 , Natalia ROȘOIU6
1 Ovidius University of Constanta, Doctoral School of Applied Sciences,
Biochemistry/Biology, Constanta, Romania, tmariavirginia@yahoo.com
2 Ovidius University of Constanta, Faculty of Pharmacy, Constanta, Romania,
Associate member of the Academy of Romanian Scientists, Bucharest, Romania, ticuta_np@yahoo.com
3 University of Bucharest, Faculty of Biology, Coresponding
member of the Academy of Romanian Scientists, Bucharest, Romania
4 Ovidius University of Constanta, Faculty of Natural Sciences and
Agricultural Sciences, Constanta,
Romania
5 Ovidius University of Constanta, Doctoral School of Applied Sciences,
Biochemistry/Biology, Constanta, Romania
6 Prof. Univ. Emeritus, Senior Researcher I, PhD, Faculty of Medicine and
Doctoral School of Applied Sciences, Biology / Biochemistry Section, Ovidius
University of Constanta, Romania, Full member of the Academy of Romanian
Scientists, Biological Sciences Section President, Bucharest, Romania
*Corresponding authors at: tmariavirginia@yahoo.com, ticuta_np@yahoo.com
Abstract. In
the biomedical sciences field of recent years, phytotherapeutic
products usage has intensified because they are a safe and sustainable
alternative, with less environmentally aggressive molecules that are able to
meet therapeutic needs. Mature vegetal product Taraxacum sp., also known as
dandelion, has been used for hundreds of years as a traditional remedy for
liver, kidney, lung, gastric diseases and even some cancers, due to its
anti-inflammatory and antioxidant effects. The aim of this paper was to assess
the contents of some bioactive principles and overall antioxidant potential of
Taraxacum sp. hydroalcoholic extracts obtained from both plant organs, herba and radix. The vegetal product was collected in the
period September-October 2021, from spontaneous flora of South Dobrudja area, Romania. Fresh plant was dried at room
temperature on metal sieves, grind to a fine powder and extracted 10%
concentration in
ethanol 50% and 70% concentrations, using cold maceration and
Soxhlet extraction, standard methods, followed by filtration at normal
pressure. Obtained hydroalcoholic extracts were analysed
by UV-Vis spectrophotometry for determining total carotenoids, flavonoids and
total polyphenols
content. The total antioxidant capacity was quantified through photochemiluminescence method by comparison with the
standard substance used for calibration, Trolox as tocopherol analogue by ACL
(Antioxidant Capacity of Lipid Soluble Substances) procedure using Photochem apparatus, Analytik
Jena AG, Germany. Total carotenoids and flavonoids concentration, respectively
polyphenols contents, were highest in 70% ethanol extracts, for the two applied
extraction methods. Total antioxidant capacity (TEAC) was variable, with
increased values in 70% ethanol extracts of both vegetal products, herba and radix. The preliminary valuable obtained results,
offer us the support for continuation of the studies regarding the therapeutic
activity of Taraxacum sp. from Dobrudja spontaneous
flora.
Keywords:
Taraxacum sp., polyphenols,
carotenoids, flavonoids, antioxidant activity.
DOI https://doi.org/10.56082/annalsarscibio.2022.1.31
